voltage dependent anion channel 1  (Proteintech)

Journal: International Journal of Oncology

Article Title: Targeting CALR reduces energy metabolism of esophageal cancer cells and inhibits tumor-associated fibroblast infiltration

doi: 10.3892/ijo.2025.5755

Figure Lengend Snippet: CALR regulates IP3R calcium ion release channels and ER stress. (A) Protein expression of (B) CALR, (C) CANX and (D) PDIA3 in KYSE150 after treatment with ER stress agonist thapsigargin. (E) Protein expression of (F) CALR, (G) CANX and (H) PDIA3 in KYSE410 cells following treatment with ER stress agonist thapsigargin. (I) In intracellular GRP78 expression after overexpression or knockdown of CALR in KYSE150. (J) Protein expression of GRP78 in KYSE150. (K) In intracellular GRP78 expression after overexpression or knockdown of CALR in KYSE410. (L) Protein expression of GRP78 in KYSE410. (M) Co-IP revealed the interaction of GRP75 and VDAC1 and IP3R1 in KYSE150. (N) Co-IP revealed the interaction of GRP75 and VDAC1 and IP3R1 in KYSE410. †† P<0.01, ††† P<0.001 vs. DMSO, ** P<0.01, *** P<0.001 vs. OE-NC, ### P<0.001 vs. sh-NC. CALR, Calreticulin; IP3R, inositol 1,4,5-Trisphosphate Receptor; ER, endoplasmic reticulum; CANX, calnexin; PDIA3, protein disulfide isomerase A3; ESCC, esophageal squamous cell carcinoma; GRP, glucose regulatory protein; VDAC, voltage-dependent anion channel; IB, immunoblotting; IP, immunoprecipitation; OE, overexpression; NC, negative control; sh, short hairpin.

Article Snippet: GRP75 (1:20,000; cat. no. 14887-1-AP), Voltage dependent anion channel 1 (VDAC1) (cat. no. 55259-1-AP, both Proteintech Group, Inc.) and inositol 1,4,5-Trisphosphate Receptor (IP3R1) (both 1:2,000; cat. no. DF3000, Affinity Biosciences).

Techniques: Expressing, Over Expression, Knockdown, Co-Immunoprecipitation Assay, Western Blot, Immunoprecipitation, Negative Control